... Sigma), followed by appropriate Ig horseradish peroxidase conjugate (Jackson ImmunoLab, West Grove, PA). The immunoreactive signal was detected by enhanced chemiluminescence (GE Healthcare, Pittsburgh, PA).
jackson immunolabs
The anti-rabbit secondary antibody (Jackson Immunolab, #711-065-152) was applied at 1:500 for 1 hr at room temperature, followed by chromogenic detection kit DABMap (Ventana Medical Systems, #760-124). Slides were& ...
Antigen binding to the beads was confirmed by staining with an Fc-specific anti-IgG-FITC antibody (Jackson Immunolaboratories, West Grove, PA, USA). This antibody did not bind to the beads in the absence of Fc-tagged& ...
... Dickinson, Franklin Lakes, NJ) as previously described [29]. Cells were treated with specific mAb or control IgG for 45 min followed by goat-anti-mouse IgG coupled with FITC (Jackson Immunolaboratory, Bar Harbor, Maine).
... Sigma), followed by appropriate Ig horseradish peroxidase conjugate (Jackson ImmunoLab, West Grove, PA). The immunoreactive signal was detected by enhanced chemiluminescence (GE Healthcare, Pittsburgh, PA).
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- Aug 28 Wed 2013 12:44
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